The detector is a necessary part of any experimental chromatography system. It is
used to determine the concentrations of the different compounds as they elute from
the column. It also provides information on the condition of the column and on the
identity of the sample components.
The output of the detector is connected to a strip chart recorder or data system.
The recorded trace becomes the chromatogram describing the separation.
The detector output can also be connected to an intelligent fraction collector
(like the CF-2)
to provide real-time peak detection and allow you to control how the components
of your precious samples are collected.
The Spectra/Chrom® Model 280 UV detector is a fixed wavelength flow-through
spectrophotometer ideally suited for use as a chromatographic detector. It works
equally well with HPLC and low-pressure systems. It features a direct reading
digital output and a dual beam optical system that allows the automatic subtraction
of a solvent background even in the presence of a rapidly changing gradient.
It has analog outputs for both a chart recorder and an integrator (1V/AU).
As with all of Spectrum's chromatography instruments, the Model 280 UV detector
comes complete and ready-to-use. It includes a 5 mm pathlength flow cell, filters
for both 254 and 280 nm operation, and a set of tubing fittings. Unlike many filter
based detector, the operating wavelength can be changed in less than 10 seconds.
The CF-2 Fraction Collector is an excellent companion to the
Model 280 UV Monitor. It allows to you to collect your separated sample components
and annotate the recorder trace to make it easy to locate your peaks.